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Figure 4 | BMC Cell Biology

Figure 4

From: Distinct genetic programs guide Drosophila circular and longitudinal visceral myoblast fusion

Figure 4

Longitudinal muscle fusion requires rols7 . Expression of bap-lacZ (green in A, B, E, F) or HLH54F-lacZ (green in G–J; white in C and D) visualized by staining with fluorescent anti-β-Gal and counterstaining with anti-β3-Tubulin (anti-β3Tub red in E, F, H; white in I). Staining of visceral mesoderm with anti-Fasciclin III (anti-FasIII, red in G and J). Lateral view of wild-type embryo (A) and rols mutant embryo (B) at stage 12. Note the β-Gal-positive cells in the rols mutant embryo (B) along the stretching circular muscles. Dorsolateral views of stage 16 wild-type embryo (E) and rols7 mutant embryo (F). (E, F) Arrowheads point to the position of the 1st midgut constriction. (C, D) rols7- deficient embryos stained with anti-β-gal showing (C) mononucleated migrating longitudinal FCs with random protrusions (arrowheads) and (D) morphology of binucleated longitudinal muscles (arrowheads). (G) Anti-FasIII staining of rols7- deficient embryos in mid-embryogenesis; longitudinal FCs located dorsally and ventrally on stretching FasIII-positive circular muscles, which sometimes display small gaps (arrowheads). (H–J) Anterior midgut regions covered with mainly mononucleated longitudinal muscles in different rols alleles at the end of embryogenesis (arrowhead); posterior midgut regions with parallel-orientated longitudinal muscles. Arrows point to regions lacking longitudinal muscles.

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