Figure 6
From: Distinct genetic programs guide Drosophila circular and longitudinal visceral myoblast fusion
scar is required for longitudinal fusion, and FC migration/fusion is promoted by wip. (A–C) Lateral view of stage 16 embryos stained with anti-β3-Tubulin to visualize gut constrictions. (A) Wild-type embryo showing normal gut constrictions (arrow). (B) Homozygous scarΔ37 single and (C) scarΔ37wipf06715 double mutant embryos showing normal gut constrictions, but aberrant gut morphology. (D, F) Homozygous scarΔ37wipf06715 double mutant embryos carrying HLH54F-GFP to mark longitudinal myogenesis. (E) Late stage 15 embryo expressing HLH54F-lacZ in a wild-type background. (D) Stage 13 embryo showing mononucleated myoblasts (arrow) and myoblasts with migration defects (arrowhead). (F) Stage 15 embryo displaying binucleated (two arrowheads) or mononucleated myoblasts (one arrowhead). (G–J) Gene dosage experiments. Embryos were stained with anti-β-Gal, anti-β3-Tubulin, and anti-FasIII. (G, H) Homozygous scarΔ37 mutant embryo carrying HLH54F-GFP and lacking one copy of wipf06715. (G) Late stage 13 embryo with normal longitudinal myoblast migration. Sometimes binucleated cells were seen (arrows). (H) Stage 16 embryo with binucleated gut muscles (arrowheads). (I, J) Homozygous wipf06715 mutant embryo carrying HLH54F-GFP and lacking one copy of scarΔ37. (I) Stage 13 embryo showing aberrant cell migrations (arrowhead) and abnormal protrusion formation (arrow). (J) Stage 15 embryo with binucleated gut muscles (arrows). Scale bars: 50 μm.