Figure 4
From: Novel Cul3 binding proteins function to remodel E3 ligase complexes
B/C-like box mediates binding between Cul3 complexes and LRRs. A. Alignment of VHL and Elongin C B/C boxes with putative B/C-like boxes in three LRRs identified in our study. B. Cul3 binding to wild type LRR5 and LRR5-B/C-like box mutant. Upper blot shows immunoprecipitation of Cul3 followed by blotting with anti-HA antibody to detect the presence of LRR5 proteins. Lower blot, relative amounts of LRR5. C. Ctb73 binding LRR5-B/C-like box mutants. Upper blot shows immunoprecipitation of Ctb73 followed by blotting with anti-HA antibody to detect the presence of LRR5 protein. Lower blots show relative amounts of LRR5 and Ctb73 proteins. D. Binding of Ctb73 and Ctb73ΔBTB mutants to LRR and LRR5-B/C mutant. Upper blot shows immunoprecipitation of Ctb73 followed by blotting with anti-HA antibody to detect the presence of LRR5 proteins. Lower blots show relative amounts of LRR5 and Ctb73 proteins. E. Graphical summary of the interactions between Cul3, a BTB domain-containing protein and a LRR domain-containing protein.