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Figure 5 | BMC Cell Biology

Figure 5

From: Novel Cul3 binding proteins function to remodel E3 ligase complexes

Figure 5

LRR5 regulates FAZF abundance. A. LRR5 was co-expressed with either PLZF (lane 2) or FAZF (lane 4). Binding was only seen with the LRR5/FAZF pair (upper blot, lane 4). Protein levels are shown in lower two blots. B. Coexpression of LRR5 and FAZF results in reduced FAZF expression (upper blot, lane 1 vs lane 2). Levels of FAZF are restored during co-expression when the proteasome is inhibited by MG-132 (lanes 3 and 4). Relative protein levels are shown in lower blots. C. LRR5 expression reduces levels of endogenous FAZF, upper blot shows LRR5 expression with increasing amounts of shRNA. Middle blot is endogenous FAZF (arrow). Lower blot is actin to show relative protein loading levels. D. Same as in C, but instead of co-expression LRR5, LRR1 was used.

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