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Figure 4 | BMC Cell Biology

Figure 4

From: IRE1 inhibition perturbs the unfolded protein response in a pancreatic β-cell line expressing mutant proinsulin, but does not sensitize the cells to apoptosis

Figure 4

Effect of MKC-3946 IRE1 inhibitor on XBP1 splicing and UPR gene induction. A. Insulin 2 C96Y-GFP cells were treated or not with 2 μg/ml Dox for 48 h in the presence or absence of 10 μM MKC-3946 and total RNA was isolated. In lanes 4 and 5 the cells were treated with 1 μM thapsigargin (Tg) for 3 h prior to RNA isolation. B. Real-time PCR was used to analyze relative levels of the indicated genes. Results are from N = 3 independent experiments.

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