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Figure 2 | BMC Cell Biology

Figure 2

From: Human ASPL/TUG interacts with p97 and complements the proteasome mislocalization of a yeast ubx4 mutant, but not the ER-associated degradation defect

Figure 2

ASPL interacts with the p97 N-domain. (a) Schematic diagram of the ASPL domain organization and the various truncations used in the precipitation experiments. (b) Purified 6His-tagged p97 was incubated with GST or the indicated GST-tagged ASPL truncations and precipitated with glutathione (GSH) Sepharose. Bound proteins were analyzed by SDS-PAGE and blotting using antibodies to the 6His-tag on p97 (upper panel). Even loading was checked by staining with Coomassie Brilliant Blue (CBB) (lower panel). (c) Schematic diagram of the p97 domain organization and the various truncations used in the precipitation experiments. (d) Purified 6His-tagged p97 and p97 truncations were incubated with GST and GST-tagged ASPL before precipitation and analysis by SDS-PAGE and blotting using antibodies specific for the 6His-tagged p97 proteins (upper panel). Even loading was checked by staining with Coomassie Brilliant Blue (CBB) (lower panel).

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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