Figure 3

Expression and localization of ASPL. (a) The rat tissues were analyzed by SDS-PAGE and blotting using antibodies specific for ASPL (upper panel) and tubulin (lower panel). ASPL was present in most tissues, but less abundant in kidney. (b) HeLa cells lysates were separated by centrifugation into a soluble (Sol.) and insoluble (Pellet) fraction and analyzed by SDS-PAGE and blotting using antibodies to the listed proteins. Calnexin served as a control for an insoluble (transmembrane) protein, while Txnl1 served as a control for a soluble protein. (c) Confocal micrographs of HeLa cells transfected to express ASPL with a C-terminal GFP-tag. The localization of ASPL in formaldehyde-fixed cells was detected with anti-GFP antibodies (green) (left panel), and compared with the localization of the ER protein calnexin (red) (middle panel). In the merged image (right panel), the signals overlap partially (yellow). The presented cells are derived from different confocal scans. Size-bar = 10 μm.