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Figure 4 | BMC Cell Biology

Figure 4

From: Human ASPL/TUG interacts with p97 and complements the proteasome mislocalization of a yeast ubx4 mutant, but not the ER-associated degradation defect

Figure 4

ASPL does not influence the degradation of model ERAD substrates. (a) MelJuSo cells were transfected with control siRNA or two different siRNAs, specific for ASPL. After 3 days, the expression of the indicated proteins was analyzed by SDS-PAGE and blotting. Actin served as a loading control. (b) MelJuSo cells expressing YFP-tagged CD3δ were analyzed in cultures treated with cycloheximide. At the indicated times the amount of CD3δ-YFP was analyzed by SDS-PAGE and Western blotting using the indicated antibodies. Antibodies to α-tubulin were used for the loading control. (c) HeLa cells expressing HA-tagged TCRα were analyzed in cultures treated with cycloheximide. At the indicated times the amount of TCRα-HA was analyzed by SDS-PAGE and Western blotting using the indicated antibodies. Antibodies to α-tubulin were used for the loading control.

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