Figure 1

Bioinformatic analysis of p49 protein sequence. A. A schematic of the p49 protein with two conserved regions. The SRF-binding domain is at the N-terminus; a BUD22 domain is located at the C-terminus. B. A BUD22 domain was found at the C-terminus of human, mouse and rat p49 proteins and three yeast BUD22 proteins, based on results from the Conserved Domain Database (NCBI), Pfam and InterPro databases. The lengths of the BUD22 domains in the six protein sequences illustrated in this diagram were based on the data from the Conserved Domain Database. According to the Conserved Domain Database, the BUD22 domain in human p49 was 29 amino acids in length. We found that this 29 amino-acid region (between two dash lines) was highly conserved in all six protein sequences. C. A schematic of the mouse p49 protein (Unipot ID Q9CZ91) which has four nuclear localization signal sequences (NLSs), and two nucleolar localization signal sequences (NoLSs). D. A schematic of the BUD22 protein of Baker’s yeast (Unipot ID Q04347), which has 3 NLSs and 3 NoLSs. E. A diagram of computational prediction of nucleolar localization signal sequence in mouse p49 protein (Unipot ID: Q9CZ91) using “NOD” (Nucleolar localization sequence Detector) website [44]. F. A diagram of computational prediction of nucleolar localization signal sequence in BUD22 of Baker’s yeast (Unipot ID Q04347) using “NOD” (Nucleolar localization sequence Detector) website [44].