Overexpression of p49/STRAP alters cellular cytoskeletal structure and gross anatomy in mice

Table 1 Bioinformatic analysis of three p49/STRAP proteins and two BUD22 proteins

	hs. p49	mm p49	Rat p49	BUD22 (B. Yeast)	BUD22 (S. Pombe)
Protein length	429 aa	441 aa	442	519 aa	388 aa
BUD22 domain	29 aa	76 aa	82aa	411 aa	362aa
% (BUD22)	6.80%	17.20%	18.50%	85%	93%
NLS	4	4	4	3	4
ER membrane retention signal	None	None	None	1	1
PTS2	1	None	None	1	none
k-NN prediction	65.2% nuclear	69.6% nuclear	69.6% nuclear	73.9% nuclear	69.6% nuclear
	21.7% cytoplasmic	4.3% cytoplasmic	4.3% cytoplasmic	26.1% cytoplasmic	13% cytoplasmic
	4.3% ER	13% cytoskeletal	13% cytoskeletal		8.7% cytoskeletal
	4.3% vesicles of secretory system	4.3% vesicles of secretory system	4.3% vesicles of secretory system		4.3% endoplasmic reticulum
	4.3% mitochondrial	4.3% plasma membrane	4.3% mitochondrial		4.3% plasma membrane
		4.3% Golgi	4.3% Golgi

Both p49 and BUD22 proteins were predicted to be present in the nucleus, but also in the cytoplasmic compartments, including mitochondria, endoplasmic reticulum, plasma membrane and vesicles of the secretory system.

ISSN: 2661-8850