Figure 6

Cellular localization of overexpressed OAS1 p42 and p46 isoforms in HeLa cells. HeLa cells were transfected with plasmids encoding OAS1 p42 or p46, as indicated. Prior to fixation the cells were treated with Mitotracker (red). The cells were incubated with the OAS1 primary antibody and a FITC-conjugated secondary antibody (green). The nuclei were stained with DAPI (blue). Yellow fluorescence indicates the degree of co-localization between the OAS1 p42 or p46 proteins and the mitotracker in the merged pictures. The frame in the merged picture indicates the magnified area. Arrows indicate some of the positions of co-localization. The white bars are 10 μm.