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Figure 2 | BMC Cell Biology

Figure 2

From: UIM domain-dependent recruitment of the endocytic adaptor protein Eps15 to ubiquitin-enriched endosomes

Figure 2

PM-GFP-Ub recruits FLAG-Eps15 to early endosomes in a UIM-domain-dependent manner. A-D. COS-7 cells transfected with FLAG-Eps15 or FLAG-Eps15-ΔUIM, together with PM-GFP or PM-GFP-Ub as indicated, were processed for IF, detecting FLAG-Eps15 constructs with anti-FLAG antibodies. E. Colocalization of FLAG-Eps15 or FLAG-Eps15-ΔUIM with EEA1 in COS-7 cells co-expressing either PM-GFP or PM-GFP-Ub was determined and is presented as in Figure 1C, averaging values for at least 10 cells in each of 3 experiments, +/- SEM. The data were compared using a one-way ANOVA test (***p < 0.0001). Scale bars; 10 μm.

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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