Leucine modulates dynamic phosphorylation events in insulin signaling pathway and enhances insulin-dependent glycogen synthesis in human skeletal muscle cells

Table 1 Results of the bounded area test

Treatment	INS			LEU			LEU + INS			LEU + INS vs. INS
Biological replicate	R1	R2	R3	R1	R2	R3	R1	R2	R3	R1	R2	R3
pAKT/AKT	7e⁻³	7e⁻⁵	4e⁻³	>0.5	>0.5	>0.5	3e⁻⁵	1e⁻⁵	2e⁻⁵	2e⁻²	1e⁻⁵	4e⁻⁶
pGSK3β/GSK3β	3e⁻⁶	2e⁻²	4e⁻²	>0.5	>0.5	>0.5	3e⁻⁴	9e⁻⁴	2e⁻²	3e⁻²	8e⁻⁴	1e⁻³
pmTOR/mTOR	>0.5	>0.5	>0.5	>0.5	3e⁻²	>0.5	>0.5	1e⁻²	2e⁻⁴	>0.5	>0.5	>0.5
pERK/ERK	>0.5	9e⁻³	4e⁻²	>0.5	>0.5	>0.5	2e⁻²	4e⁻²	2e⁻²	2e⁻²	4e⁻²	2e⁻²
pp70S6K/p70S6K		4e⁻²						3e⁻²			8e⁻⁵
p4EBP1/4EBP1	>0.5	>0.5	>0.5	>0.5	>0.5	>0.5	6e⁻⁴	2e⁻²	2e⁻²	>0.5	>0.5	>0.5
pFOXO1/FOXO1			>0.5			>0.5			1e⁻²			>0.5

Results of the Bounded Area method [13], applied to the time courses of the ratio between phosphorylated and total proteins monitored in a single replicate, to identify, for each protein, the replicates whose overall dynamic profile is significantly modulated by insulin, leucine and/or leucine + insulin treatment. False Discovery Rate corrected p-values are reported. Empty cells indicate that data are not available.

ISSN: 2661-8850