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Table 1 Results of the bounded area test

From: Leucine modulates dynamic phosphorylation events in insulin signaling pathway and enhances insulin-dependent glycogen synthesis in human skeletal muscle cells

Treatment INS LEU LEU + INS LEU + INS vs. INS
Biological replicate R1 R2 R3 R1 R2 R3 R1 R2 R3 R1 R2 R3
pAKT/AKT 7e−3 7e−5 4e−3 >0.5 >0.5 >0.5 3e−5 1e−5 2e−5 2e−2 1e−5 4e−6
pGSK3β/GSK3β 3e−6 2e−2 4e−2 >0.5 >0.5 >0.5 3e−4 9e−4 2e−2 3e−2 8e−4 1e−3
pmTOR/mTOR >0.5 >0.5 >0.5 >0.5 3e−2 >0.5 >0.5 1e−2 2e−4 >0.5 >0.5 >0.5
pERK/ERK >0.5 9e−3 4e−2 >0.5 >0.5 >0.5 2e−2 4e−2 2e−2 2e−2 4e−2 2e−2
pp70S6K/p70S6K   4e−2       3e−2    8e−5  
p4EBP1/4EBP1 >0.5 >0.5 >0.5 >0.5 >0.5 >0.5 6e−4 2e−2 2e−2 >0.5 >0.5 >0.5
pFOXO1/FOXO1    >0.5    >0.5    1e−2    >0.5
  1. Results of the Bounded Area method [13], applied to the time courses of the ratio between phosphorylated and total proteins monitored in a single replicate, to identify, for each protein, the replicates whose overall dynamic profile is significantly modulated by insulin, leucine and/or leucine + insulin treatment. False Discovery Rate corrected p-values are reported. Empty cells indicate that data are not available.