Biochemical changes in resveratrol-treated BPAEC. BPAEC were treated with the indicated concentrations of resveratrol and lysed after 6, 48, and 96 h, respectively. Lysates were run under 10% SDS-PAGE, and probed with antibodies for the various target proteins. Panel A. Changes in activated ERK1/2-P (representative of 3 experiments). Panel B. Changes in ERK1/2 (representative of 2 experiments). Panel C. Changes in eIF-4E. Panel D. Changes in eNOS. Panel E. Changes in actin. Lanes 1,4,7 correspond to control at 6, 48, and 96 h; lanes 2,5,8 correspond to BPAEC treated with 25 μM resveratrol for 6, 48, and 96 h; lanes 3,6,9 correspond to BPAEC treated with 100 μM resveratrol for 6, 48, and 96 h. The results represent the average of two experiments, each analyzed in duplicate or triplicate.