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Figure 5 | BMC Cell Biology

Figure 5

From: Biochemical, electron microscopic and immunohistological observations of cationic detergent-extracted cells: detection and improved preservation of microextensions and ultramicroextensions

Figure 5

Double staining of actin and moesin in NIH3T3 cells fixed with DOTMAC/PFA shown at high magnification. Note that branched micropodia were well preserved by the DOTMAC/PFA fixative and stained with both β-actin monoclonal and moesin polyclonal antibodies. Some of the moesin-positive micropodia were β-actin-negative (arrowhead). Bars, 50 μm.

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