Figure 1
Effect of pertussis toxin (PTX) on ERK1/2 activation. PTX was added to cultured hepatocytes at 3 h after the time of seeding. A: ERK1/2 activation in the absence or presence of PTX (400 ng/ml) in response to stimulation with PGF2α (10 μM) for 5 min in the period from 8 h to 48 h. B, C: Effect of PTX (300 ng/ml) on ERK1/2 activation in response to stimulation with EGF (10 nM) (B) or HGF (100 ng/ml) (C) for 5 min at 24 and 48 h. D: Time-course for HGF- (100 ng/ml) induced ERK1/2 activation in the absence or presence of PTX (300 ng/ml). Results in A-D represent means ± S.E.M. from three experiments.