Blocking of cell attachment to III1-C RASMCs were seeded onto wells coated with either 20 μg/ml FN (top panel), or 25 μM III1-C (bottom panel) in the absence or presence of various inhibitors. The inhibitors were 100 μg/ml heparin (Hep), 10 mM EDTA (EDTA), 50 μg/ml anti-β1 blocking mAb Ha2/5 (pi), 0.5 mg/ml GRGDSP peptide (RGD), 0.5 mg/ml GRADSP peptide (RAD), 100 μg/ml anti-FN polyclonal antibody (anti-FN), and 60 μg/ml anti-IIIl-C polyclonal antibody (anti-C). After 30 min at 37°C the cells were fixed, stained with crystal violet and the amount of cell attachment was measured. The dashed line indicates the amount of signal seen on the negative control protein III 11-C, and represents the background A600 reading in these experiments. Statistical analysis was done by Student's T-test (2-tailed); * p < .05, ** p < .005, *** p < .0005. Each sample was done in quadruplicate and the experiment was performed three times with similar results each time.