Figure 6
From: Spatial partitioning of secretory cargo from Golgi resident proteins in live cells
Post-Golgi carriers can be as large as the Golgi elements from which they originate Transiently expressed VSVG3-YFP cargo was accumulated in the ER and pulsed into the secretory pathway of PtK2 cells as in Figure 1B. Time at 32°C and the frame number of the corresponding movie are indicated (Additional Fileset 6). Cargo and Golgi resident coincide in a discrete Golgi element (1). A cargo domain forms (2) which extends (3), rapidly pulls off of the Golgi element (4), and recondenses (5 to 7) to a spherical TC of a size similar to the original Golgi element. The TC translocates away from the Golgi element (8) as another large TC enters the field of view (9), and appears to interact with the element (Additional Fileset6). The first TC continues to translocate away (10). A nearby Golgi element (12) has a cargo domain (13) which displays obvious coincident dynamics. Bar: 10 μm.