Figure 3

Colocalization of β-dystroglycan and von Willebrand factor in normal mouse forebrain. Shown are cryosections indirectly labeled with rhodamine-conjugated antibodies to the neurofilament of apparent 68 kDa (a), the glial fibrillary acidic protein (b, c) and von Willebrand factor (d). Sections (a) to (d) were indirectly double-labeled with a fluorescein-conjugated antibody against β-dystroglycan. To demonstrate the specificity of the antibody to von Willebrand factor, rat aorta sections are shown in (e) (Haematoxylin & Eosin staining) and (f) (immunofluorescence labeled). In (a), bar = 20 μm; in (b) and (d), bar = 40 μm; in (c), bar = 10 μm; and in (e) and (f), bar = 60 μm.