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Table 1 Changes of intracellular Ca2+ concentration in the cell population with elevated [Ca2+]i, assessed by flow cytometry using Fluo-3fluorescence (arbitrary units) in control and Scott cells after 48 or 72 h of apoptosis induction by calcium ionophore A23187 (200 nM) or/and SKF 96365 (10 μM), in the absence and presence of the caspase inhibitor Ac-DEVD-CHO (50 μM). Values are the mean ± SEM of 4 independent experiments.

From: Deciphering the plasma membrane hallmarks of apoptotic cells: Phosphatidylserine transverse redistribution and calcium entry

CELLS No treatment A23187 (200 nM) SKF 96365 (10 μM) A23187 + SKF 96365 Ac-DEVD-CHO + A23187
Control (48 h) 398 ± 25 545 ± 51** 409 ± 17 524 ± 25* 522 ± 23*
Scott (48 h) 371 ± 5 382 ± 1 390 ± 7 371 ± 14 380 ± 10
Control (72 h) 361 ± 22 470 ± 25* 417 ± 31 490 ± 44* 520 ± 90*
Scott (72 h) 403 ± 62 408 ± 70 376 ± 46 354 ± 54 356 ± 43
  1. *P < 0.05, **P < 0.01 versus the corresponding values obtained with non-treated cells.