Figure 2

Conventional electron micrographs of thin sections of liver mitochondria assayed the quality of tBid_G94E labelling. a) Section of pelleted mitochondria decorated with immunogold tBid_G94E. Most of the mitochondria are in the condensed state [33]. No difference in the general number of gold particles between condensed and orthodox mitochondria was observed. Peroxisome contamination indicated by asterisks in (a) and (c) had no immunogold background labeling.b) Mitochondrion boxed in (a) magnified 3x in order to visualize the 5-nm gold particles (arrowheads). c) No-primary control section showing little, if any, nonspecific immunogold labeling on mitochondria treated with gold-conjugated secondary antibody.d) Mitochondrion boxed in (c) magnified 3x in order to show that there is no gold around the perimeter. Scale bars: (a), (c), 1000 nm.