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Figure 1 | BMC Cell Biology

Figure 1

From: The linkage between β1 integrin and the actin cytoskeleton is differentially regulated by tyrosine and serine/threonine phosphorylation of β1 integrin in normal and cancerous human breast cells

Figure 1

Coprecipitation of actin with β1 integrin in HBE cells and not in MCF-7 cells. (A) Integrin β1 was immunoprecipitated (IP) with the anti-β1 integrin antibody or control IgG from HBE or MCF-7 cells which were quiescent and adherent to collagen IV. The immunoprecipitates were immunoblotted with anti-actin or anti-integrin β1 monoclonal antibody. Molecular size markers are indicated at right in kDa. (B) Whole cell lysates were prepared from HBE or MCF-7 cells which were quiescent and adherent to collagen IV, and equal amounts (5 μ g protein) of the lysates were resolved by SDS-PAGE and blotted. The blots were probed with anti-actin or anti-integrin β1 monoclonal antibody. Molecular size markers are indicated at right in kDa.

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