Figure 1

The effect of temperature (A) and osmotic pressure (B) on spore germination. Plc-null (closed symbols) and wild-type control (open symbols) spores were inoculated in growth medium at 22°C (circles) or 16°C (triangles), or in growth medium containing 0.2 M sucrose (diamonds). The time at which 50% of the spores were germinated was calculated by exponential curve fitting using the program FigP. The data shown are from a typical experiment; the calculated germination time for many different conditions are summarized in table 1.