Skip to main content

Table 1 Spore germination at different environmental conditions

From: Phospholipase Cδ regulates germination of Dictyostelium spores

Condition

50% germination (hours)

Significance

 

wild-type

plc-null

 

Temperature (°C)

   

10

>250

16.9 ± 0.6

-

13

38 ± 7

7.1 ± 2.0

**

16

17.4 ± 2.6

5.1 ± 0.4

**

19

7.0 ± 0.5

4.3 ± 1.1

*

22 #

4.3 ± 0.3

4.2 ± 0.2

NS

Osmolarity (M)

   

0 #

4.3 ± 0.3

4.2 ± 0.2

NS

0.10

5.5 ± 1.1

4.6 ± 0.4

NS

0.15

10.0 ± 0.7

5.2 ± 0.9

**

0.20

17.8 ± 1.2

6.3 ± 1.4

**

0.30

30.9 ± 3.1

9.0 ± 1.0

**

pH

   

3.0

>250

>250

-

4.0

>250

>250

-

4.7

127 ± 21

88 ± 18

NS

5.2

19.7 ± 2.6

16.8 ± 2.6

NS

5.5

7.6 ± 2.4

9.4 ± 3.1

NS

6.2

3.6 ± 1.6

4.2 ± 1.7

NS

7.1 #

4.3 ± 0.3

4.2 ± 0.2

NS

7.5

5.2 ±1.5

4.6 ± 1.4

NS

7.8

5.6 ±1.0

5.0 ± 1.3

NS

8.4

8.2 ± 0.9

5.7 ± 0.8

*

9.0

20.6 ± 3.4

9.0 ± 1.1

**

11

>250

>250

-

  1. Spore germination was measured at different temperatures, increased osmolatity by added glucose or at different pH of the medium; #, normal medium. Data are the means and standerd deviations from three independent experiments. NS, the difference between wild-type and plc-null is not significant at P > 0.05; * and **, significant at P < 0.05 and P < 0.01, respectively.