| | Normal cortex | Normal cortex | Abnormal | Abnormal | Total number of |
|---|
| | | | cortex | cortex | cells observed |
|---|
| | Normal oral | Abnormal oral | | | |
|---|
| | apparatus | apparatus | Normal oral | Abnormal oral | |
|---|
| | | | apparatus | apparatus | |
|---|
Number of cells | 6 | 40 | 9 | 87 | 142 |
With erratic | 0 | 3 | 4 | 12 | 19 |
microtubules | | | | | |
With internal | 0 | 0 | 6 | 30 | 36 |
basal bodies | | | | | |
- 48 hours after microinjection, i.e. after microinjected cells had undergone 5-6 divisions, a random sample was taken from 26 transformed clones, pooled and processed for immunofluorescence. Labelling with the anti-tubulin ID5 [19] reveals the basal body pattern on the cortex and in the oral apparatus allowing detection of even small pattern abnormalities and of "internal basal bodies", not inserted in the cortex or in the oral structure, never observed in normal cells. In addition to basal bodies, the monoclonal ID5 stains the thick post-oral fibers and the proximal part of the microtubule rootlets of the vacuole pore system (see Figure 2), but no other microtubule of the internal microtubule network. Cells were classified according to cell size, to defects in either cortex or oral apparatus or both and to the presence/absence of internal basal bodies and erratic intracytoplasmic microtubules (see text and Figure 2). The figures indicate the number of cells in each category.
