Figure 1
Effect of L-T 4 on [14C]DAG and [14C]phospholipid level in liver slices and isolated hepatocytes. [14C]sodium acetate labeled liver slices (A) and [14C]oleic acid labeled hepatocytes (B) were treated with 100 nM NaOH (control) or 10 nM L-T4 for indicated times. Lipids were extracted, separated by TLC, and analyzed as described in "Materials and Methods". Basal lipid levels in control cells (Fig. 1B) did not change significantly over the experimental period and were (cpm/107 cells): PI -4721 ± 633, PI(4)P - 4265 ± 264, PI(4,5)P2 - 3605 ± 317, PC - 7662 ± 344, DAG - 6008 ± 106. Results represent the mean ± S.E. of four individual experiments.* P < 0.05 vs. control.