Thyroxine signal transduction in liver cells involves phospholipase C and phospholipase D activation. Genomic independent action of thyroid hormone

Table 2 Effect of H7 on the L-T₄-induced DAG and Peth formation in [¹⁴]oleic acid pre-labeled hepatocytes.

Lipid	Additions	Control	L-T₄	% of control
		cpm/1 0⁷ cells
	NaOH+ethanol	2170 ± 362	5000 ± 638^*	230
[¹⁴C]Peth
	H7 + NaOH + ethanol	1847 ± 296	2915 ± 44^*	158
	NaOH	10797 ± 715	16515 ± 1077^*	153
[¹⁴]DAG
	H7+NaOH	7359 ± 746	9051 ± 1290	123

[¹⁴C]oleic acid labeled hepatocytes were pre-incubated for 15 min with H7 (50 μM) or NaOH (l00 nM) alone prior to stimulation with L-T₄ for 2 min. 1.5% ethanol was added 15 min before L-T₄ addition or together with H7 (50 μM) or NaOH (100 nM) for determination of Peth formation in the cells. The data are mean ± S.E. of four separate experiments. ^* P < 0.05 vs. control.

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