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Table 2 Effect of H7 on the L-T4-induced DAG and Peth formation in [14]oleic acid pre-labeled hepatocytes.

From: Thyroxine signal transduction in liver cells involves phospholipase C and phospholipase D activation. Genomic independent action of thyroid hormone

Lipid Additions Control L-T4 % of control
   cpm/1 07 cells  
  NaOH+ethanol 2170 ± 362 5000 ± 638* 230
[14C]Peth     
  H7 + NaOH + ethanol 1847 ± 296 2915 ± 44* 158
  NaOH 10797 ± 715 16515 ± 1077* 153
[14]DAG     
  H7+NaOH 7359 ± 746 9051 ± 1290 123
  1. [14C]oleic acid labeled hepatocytes were pre-incubated for 15 min with H7 (50 μM) or NaOH (l00 nM) alone prior to stimulation with L-T4 for 2 min. 1.5% ethanol was added 15 min before L-T4 addition or together with H7 (50 μM) or NaOH (100 nM) for determination of Peth formation in the cells. The data are mean ± S.E. of four separate experiments. * P < 0.05 vs. control.