Table 1 Buffers used in the Pseudo-Null Point pH determination experiment.
Buffer | Composition | Pseudo-Null pH |
|---|---|---|
(BA=butyric acid; TA=trimethyl amine) | ||
S1 | 3 mM BA/48 mM TA in SPB buffer, pH 7.0 | 7.6 |
S2 | 3 mM BA/12 mM TA in SPB buffer, pH 7.0 | 7.3 |
S3 | 3 mM BA/3 mM TA in SPB buffer, pH 7.0 | 7.0 |
S4 | 12 mM BA/3 mM TA in SPB buffer, pH 7.0 | 6.7 |
S5 | 48 mM BA/3 mM TA in SPB buffer, pH 7.0 | 6.3 |
S6 | SPB buffer, pH 7.0 | low osmolarity control |
S1S | S 1/400 mM sorbitol | 7.6 |
S2S | S2/400 mM sorbitol | 7.3 |
S3S | S3/400 mM sorbitol | 7.0 |
S4S | S4/400 mM sorbitol | 6.7 |
S5S | S5/400 mM sorbitol | 6.3 |
S6S | S6/400 mM sorbitol | high osmolarity control |