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Figure 4 | BMC Cell Biology

Figure 4

From: Cell density related gene expression: SV40 large T antigen levels in immortalized astrocyte lines

Figure 4

Flow cytometry measurements of Tag and DNA. This is an example of the cytometric data collected for this study. P0-3D tkT#5 cells were immunofluorescently labeled with PAB 416 (anti-Tag) or IgG2a (isotype control) and FITC-conjugated goat anti-mouse secondary antibody. DNA was stained with propidium diiodide. Mean linear immunofluorescence of the G1 sub-population for the isotype control (Fb) was subtracted from that of the Tag-stained sample (Ft) to generate the measurement of Tag expression (Fs).

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