Figure 4

Release of annexin V-binding membrane microparticles in subconfluent, confluent and overgrown HUVEC culture treated with camptothecin HUVEC at 24 hrs (50% of confluence), 48 hrs (reaching confluence) or 72 hrs (overgrown) in culture were incubated for 24 hrs with 0.5 % DMSO, 500 nM or 5 μM CPT in medium. After incubation supernatant from cell culture was harvested and annexin V-FITC-binding MP were counted. Means of 4 experiments ± SD are presented, significant differences (p < 0.05, ANOVA): 500 nM CPT – 24 hrs vs. 48 hrs, 48 hrs vs. 72 hrs, 24 hrs vs. 72 hrs; 5 μM CPT – 24 hrs vs. 48 hrs, 24 hrs vs. 72 hrs; 24 hrs – 500 nM CPT vs. 5 μM CPT; 72 hrs – 500 nM CPT vs. 5 μM CPT.