Figure 3
From: Characterization of subcellular localization and stability of a splice variant of G alphai2
Localization of sα i2 to Golgi and ER membranes sαi2 was expressed in COS-7 cells. 48 h after transfection, cells on coverslips were fixed and processed for immunofluorescence. Co-localization of sαi2 (A) and the Golgi protein, β-COP (B), was visualized after incubation of cells with the EE antibody and a rabbit polyclonal anti-β-COP antibody followed by Alexa 594 anti-mouse and Alexa 488 anti-rabbit antibodies. Co-localization of sαi2 (C) and the endoplasmic reticulum protein, protein disulfide isomerase (PDI) (D), was visualized after incubation of cells with the EE antibody and a rabbit polyclonal anti-PDI antibody followed by Alexa 594 anti-mouse and Alexa 488 anti-rabbit antibodies.