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Figure 6 | BMC Cell Biology

Figure 6

From: The CXC chemokine cCAF stimulates precocious deposition of ECM molecules by wound fibroblasts, accelerating development of granulation tissue

Figure 6

Effect of cCAF on collagen levels. (A&B) Immunoblot analysis for Colls I and III, respectively. All lanes contain equal amounts of total protein, as measured by the DC protein assay (BioRad). Treatment of fibroblasts with cCAF, but not the C- or N-peptides, stimulates a small but reproducible increase in protein levels of Coll I when compared with untreated cells (A). Neither cCAF nor peptide treatments changed Coll III levels (B). (C) Levels of Colls I and III in wounds treated with cCAF. Immunoblot analysis of protein extracts from wounded wings treated with 1 μg of cCAF were performed as described in Materials and Methods section. Coll I levels are slightly higher than those of the control after 3 days of treatment with cCAF. However, they are similar to those of the control at 7 days. Coll III levels do not differ between control and cCAF-treated wings.

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