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Figure 8 | BMC Cell Biology

Figure 8

From: The CXC chemokine cCAF stimulates precocious deposition of ECM molecules by wound fibroblasts, accelerating development of granulation tissue

Figure 8

Effect of cCAF on fibroblast migration (A) Using Transwell membrane assays we determined that treatment of fibroblasts with cCAF for 24 hours resulted in increased migration toward the chemokine. This effect is dose-dependent with an increase detected at doses of 100 ng/ml and strongest at a dose of 1000 ng/ml. Serum-containing medium was used as positive control. (B) When fibroblasts were treated with 1000 ng/ml cCAF in both wells migration to the upper side of the membrane was abrogated. (C) Fibroblasts plated inside a cloning ring. Cells were allowed to migrate away from the edges of the ring for 2 days while treated with 100–1000 ng/ml cCAF. Treatment with cCAF resulted in a significant increase in the distance migrated by the fibroblasts (* = p < 0.05; n = 4). (D&E) In situ hybridization shows that the fibroblasts migrating away form the edge express cCAF. Dash-dot lines show the edge of the culture after scraping. In (D) arrowheads point to cells labeling with the antisense probe for cCAF whereas in (E) they point to scattered labeling over the cells incubated with the sense probe.

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