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Figure 3 | BMC Cell Biology

Figure 3

From: Negative regulation of mitochondrial VDAC channels by C-Raf kinase

Figure 3

C-Raf inhibits the release of cytochrome c from mitochondria and caspases processing. 32Dcl.3 transfected cells were maintained without IL-3 for different time periods before isolating mitochondria and cytosol. Immunoblot analysis was performed on mitochondrial extracts for the detection of cytochrome c release and on cytosol for the analysis of procaspases 3 and 9 cleavage. Blots were stripped and reprobed with anti-VDAC or anti-actin antibodies as protein loading control.

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