Figure 6

Colony-sectoring analysis to test the ability of different mutant versions of Boi1 and Boi1-PH to substitute in function for wild-type Boi1 and Boi2. Values are percentages of colonies that displayed each of the different degrees of sectoring: "none" = no sectoring (i.e., thoroughly red colony), "poor" = one or a few white sectors in an otherwise thoroughly red colony, "moderate" = more than a few white sectors, but where not more than approximately half of the colony contains sectors, and "heavy" = most or all of the colony contains white sectors. The circles illustrate these categories of sectoring, with black representing the red parts of colonies. In this analysis, boi1 boi2 strain PY967, which contains wild-type BOI1 and the color marker ADE3 on one plasmid (pPB799), was transformed with a second plasmid containing the indicated construct that was being tested for function. Transformation plates were incubated at 23 or 30°C, as indicated. The different sets of amino-acid substitutions are indicated in parentheses. "Boi1" = full-length Boi1; "PH" = Boi1-PH; and "Myr-" = fusion to the myristoyl group-accepting sequence MGCTVS. The constructs were encoded by the following plasmids: - (pPB1306), Boi1 (pPB2003), Boi1(TK) (pPB2008), Boi1(KK) (pPB2002), Boi1(KKTK) (pPB1671), PH (pPB1695), Myr-PH (pPB1498), PH(TK) (pPB1669), Myr-PH(TK) (pPB1667), PH(KK) (pPB1697), Myr-PH(KK) (pPB1500), PH(KKTK) (pPB1757), and Myr-PH(KKTK) (pPB1661).