(A) The mature IGF-I domain is not required for nucleolar localization, while replacing the C-terminal part of the E domain encoded by exon 5 results in loss of specific nucleolar localization. The left panels are phase images except for 2-G-4-5 which was saved as a pseudo-DIC image instead. (B) Western blot of lysates from transfected cells using an anti-GFP antibody. The identity of the transfected chimera is given above the lanes. Positions of the size markers are given on the left.