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Figure 2 | BMC Cell Biology

Figure 2

From: A p130Cas tyrosine phosphorylated substrate domain decoy disrupts v-Crk signaling

Figure 2

Structure of attenuated Src kinase domain/Cas chimeras. The Src/Cas(SD) chimeras were constructed as described in the materials and methods. Src* is the isolated attenuated Src kinase domain (Y416F). Src*/Cas(SD) is a fusion of the attenuated Src kinase domain to a.a. L157 to R516 of the Cas substrate domain. Src*/Cas(5'SD) is a fusion of the attenuated Src kinase domain to the 5' portion of the Cas substrate domain (a.a. L157 to A324). Src*/Cas(3'SD) is a fusion of the attenuated Src kinase domain to the 3' portion of the Cas substrate domain (a.a. S325 to R516). SrcKM is the isolated inactive kinase domain (K295M). SrcKM/Cas(5'SD) is a fusion of the inactive Src kinase domain to the 5' portion of the Cas substrate domain (a.a. L157 to R516). SrcKM/Cas(3'SD) is a fusion of the inactive Src kinase domain to the 3' portion of the Cas substrate domain (a. a. S325 to R516).

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