Comparison of GFP-MHCK-C distribution patterns in the interphase of Ax2 (C) and myosin II null (C, M null) cells. In the absence of myosin II, GFP-MHCK-C does not localize to the cell cortex (C, M null, top). A line-scan of the fluorescent intensity profiles across the cells also indicates no cortical distribution in the absence of myosin II (C, M null, middle), the units of x- and y-axis are the same as in Figure 1. In moving cells, direction indicated by arrow, GFP-MHCK-C expressed in the presence of myosin II enriches at the posterior region (C, bottom), GFP-MHCK-C expressed in the myosin II null cells does not stay at the posterior of the cells (C, M null, bottom). The scale bar is 5 μm.