Figure 2

Effect of cytokines on P-gp cellular content in mature astrocytes. a/ Addition of 100 and 250 ng/ml rCNTF during 24 h induced a statistically significant increase as compared to untreated controls (+51.7% ± 15.6, factorial ANOVA significant at 95%, t-test p < 0.01^** and +75.7 ± 30, factorial ANOVA significant at 95%, t-test p < 0.05^*, respectively). b/ Addition of rhLIF during 24 h induced a significant increase of P-gp expression both at 10 and 30 ng/ml (+42.7 % ± 14, factorial ANOVA significant at 95%, t-test p < 0.05^* and +54.35 % ± 12, factorial ANOVA significant at 95%, t-test p < 0.01^** respectively). The same pattern of regulation was observed with CT-1 at 10 ng/ml (+69.6 % ± 24, factorial ANOVA significant at 95%, t-test p < 0.01^**). c/ Addition of rmIL-6 elicited a significant increase of P-gp intracellular content at 40 ng/ml (+68.8 % ± 10, factorial ANOVA significant at 95%, t-test p < 0.001^***)