Figure 8
From: Phorbol ester induces elevated oxidative activity and alkalization in a subset of lysosomes
Effect of pH on the fluorescence of oxidized OxyBURST Green H 2 HFF BSA. Oxidized OxyBURST Green H2HFF BSA (■) and 5-(and -6)-carboxyfluorescein (◆) were prepared in MES buffer solutions at various pHs, to a final concentration of 78 μg/ml and 4.2 μM respectively. The ratio of emission fluorescence at λ535 upon excitations with λ485 over λ440 (i.e. F535(λ485)/F535 (λ440)) was then determined.