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Table 1 Effect of PMA on the oxidative activity and pH of total lysosomes

From: Phorbol ester induces elevated oxidative activity and alkalization in a subset of lysosomes

 

Oxidative activitya (gray level/pixel area)

pHb

Control

82.1 ± 14.4 (100%)

4.37 ± 0.05

PMA

123.9 ± 20.3 (150%)

4.70 ± 0.21

  1. a. Total lysosomal population was first labeled with Cascade Blue-conjugated dextran (10 K) and OxyBURST Green H2HFF BSA. The fluorescent intensities of oxidized OxyBURST in lysosomes of both control and PMA-treated cells were then measured as described. b. Lysosomal pH was measured using fluorescein-dextran in a dual-excitation ratiometric mode as described in Materials and method section.
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BMC Molecular and Cell Biology

ISSN: 2661-8850

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