Figure 1From: The F-box protein SKP2 mediates androgen control of p27 stability in LNCaP human prostate cancer cellsMIB induces G1 cell cycle arrest and inhibition of CDK2 activity (A) LNCaP cells were grown in the absence or presence of 10 ng/ml MIB and harvested after the times indicated. Cell numbers were determined by counting in a hemacytometer. (B) LNCaP cells were maintained in the absence or presence of 10 ng/ml MIB for the indicated times, and cells were harvested for flow cytometry. (C) Cyclin E immunoprecipitates were retrieved from LNCaP cells treated with 10 ng/ml MIB for 72 h and examined for associated H1 kinase activity in vitro. The precipitated amount of cyclin E (top) and the associated kinase activity (H1-P) are shown (bottom). (D) Cell lysate was prepared from LNCaP cells maintained in the absence or presence of 10 ng/ml MIB for 72 h. Lysates were precipitated with cyclin E antibodies and immunoprecipitates were examined for co-precipitation of p27 by immunoblotting (lanes 1 and 2). The asterisk denotes the immunoglobulin heavy chains. Total cell lysates are shown in lanes 3 and 4.Back to article page