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Figure 1 | BMC Cell Biology

Figure 1

From: The F-box protein SKP2 mediates androgen control of p27 stability in LNCaP human prostate cancer cells

Figure 1

MIB induces G1 cell cycle arrest and inhibition of CDK2 activity (A) LNCaP cells were grown in the absence or presence of 10 ng/ml MIB and harvested after the times indicated. Cell numbers were determined by counting in a hemacytometer. (B) LNCaP cells were maintained in the absence or presence of 10 ng/ml MIB for the indicated times, and cells were harvested for flow cytometry. (C) Cyclin E immunoprecipitates were retrieved from LNCaP cells treated with 10 ng/ml MIB for 72 h and examined for associated H1 kinase activity in vitro. The precipitated amount of cyclin E (top) and the associated kinase activity (H1-P) are shown (bottom). (D) Cell lysate was prepared from LNCaP cells maintained in the absence or presence of 10 ng/ml MIB for 72 h. Lysates were precipitated with cyclin E antibodies and immunoprecipitates were examined for co-precipitation of p27 by immunoblotting (lanes 1 and 2). The asterisk denotes the immunoglobulin heavy chains. Total cell lysates are shown in lanes 3 and 4.

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