Figure 10

Localization of ubiquitin and Hsp70 in Rab24(D123I)-induced inclusions. HEK293 cells expressing mycRab24(D123I) were processed for immunofluorescence microscopy 24 h after transfection. In all panels, the myc-tagged Rab protein was detected with polyclonal antibody against the myc epitope followed by FITC-conjugated GAR IgG. Cells were co-stained with mouse monoclonal antibodies against (A) ubiquitin, (B) Hsp70/Hsc70 or (C) Hsp90, followed by rhodamine-conjugated GAM IgG. Non-transfected cells (no myc staining above background) are marked with asterisks in each panel. The bar represents 10 microns.