Figure 5

Accumulation of Rab24(D123I)-positive intranuclear inclusions is not accompanied by chromosomal DNA fragmentation. 293 cells expressing mycRab24(D123I) were fixed 24 h after transfection and subjected to the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling reaction (TUNEL). The transfected cells were identified by co-staining with anti-myc monoclonal antibody followed by rhodamine-conjugated GAM IgG. (A) Representative cells with mycRab24(D123I)-positive nuclear inclusions exhibit varying degrees of nuclear disruption but are uniformly negative when subjected to the TUNEL reaction. (B) Parallel cultures of non-transfected 293 cells were treated with 1 μM staurosporine for 24 h to induce apoptosis. Examples of TUNEL-positive cells are shown at the same exposure setting used to photograph the TUNEL-negative cells in panel A. Nuclei were visualized by staining with propidium iodide (PI).