Figure 7
Inclusions generated by Rab24(D123) contain importin-β, but not Ran binding protein. (A) mycRab24(D123I) expressed in transfected NIH 3T3 cells was detected with rabbit antibody against the myc epitope followed by FITC-conjugated GAR IgG. Importin-β was detected with mouse monoclonal antibody followed by rhodamine-conjugated GAM IgG. The lines point to aggregates staining for both myc and importin-β. (B) mycRab24(D123I) was detected as in A, and Ran BP was detected with a monoclonal antibody, followed by rhodamine-conjugated GAM IgG. The scale bars represent 10 microns.