Figure 9

Localization of enhanced green fluorescent protein (EGFP) Rab24 fusion proteins. HEK293 cells were transfected with pCMV5 expression vectors encoding: (A) EGFP fused to full-length (fl) Rab24(D123I), (B) EGFP fused to the Rab24 C-terminal hypervariable domain (ct), or (C) EGFP alone. Cells were processed for immunofluorescence microscopy 24 h after transfection. The EGFP proteins were localized with a monoclonal antibody against green fluorescent protein (GFP) followed by FITC-conjugated GAM IgG. The Rab24 sequence was detected in the same cells with a rabbit polyclonal antibody against the C-terminal domain, followed by rhodamine-conjugated GAR IgG. The bar represents 10 microns.