Rapid degradation of dominant-negative Rab27 proteins in vivo precludes their use in transgenic mouse models

Table 3 Transgenic protein and mRNA expression.

Rab27 mutant	line	mRNA expression*	transgenic protein expression
Rab27a^T23N	T27aT2	0.20 ± 0.02	not detectable
	T27aT15	0.29 ± 0.03	not detectable
	T27aT17	0.31 ± 0.01	not detectable
	T27aT20	0.29 ± 0.01	not detectable
	A27aT6	2.6 ± 0.2	++
	A27aT6/18	not determined	++
	A27aT13	1.8 ± 0.1
	A27aT25/1	0.21 ± 0.03
	A27aT25/2	1.7 ± 0.1	+++
Rab27a^Q78L	T27Q102	0.32 ± 0.06
	A27aQ13	not determined	§
	A27aQ24	not determined	§
Rab27a^N133I	T27aN14/5	0.61 ± 0.02	not detectable
	T27aN48	0.26 ± 0.02	not detectable
	A27aN2	0.95 ± 0.04	+
	A27aN6	1.27 ± 0.08	+
	A27aN14	2.2 ± 0.4	not detectable
Rab27a wt	A27aTwt2	not determined	+++
Rab27b^T23N	A27bT40	1.21 ± 0.03	+
	A27bT59	0.6 ± 0.1	not detectable
Rab27b^N133I	A27bN9	1.64 ± 0.06	++

+++ represents transgenic Rab27 protein level similar to endogenous, ++ or + represent transgenic Rab27 protein level below endogenous, §very high levels of transgenic protein compared to endogenous; * mRNA Rab27 values are expressed as (endogenous + transgenic Rab27a or Rab27b) / Hprt. Endogenous Rab27a/Hprt = 0.22 ± 0.01. Endogenous Rab27b/Hprt = 0.12 ± 0.01.

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