Figure 2From: Tracing Myelin Protein Zero (P0) in vivo by construction of P0-GFP fusion proteinsFluorescence microscopy of a S2 cell transfected with the Ala221fs mutation in fusion with GFP (vector pEXII). None of the different layers analysed by fluorescence microscopy showed a membrane insertion of P0-GFP. Fluorescent cytoplasmic vesicles (arrows) are clearly visible. The nucleus is free of fluorescence (see fig. 4).Back to article page