Figure 7
From: Tracing Myelin Protein Zero (P0) in vivo by construction of P0-GFP fusion proteins
Western blot analysis of P0 protein levels with antibodies directed against the V5-epitope. The V5 epitope fused in-frame with P0 reveals expected molecular weights for GFP and GFP fused with P0 proteins.Lane 1: purified recombinant GFP.Lane 2: crude protein extract of sciatic nerve.Lane 3: protein extract of GFP-V5 transfected insect cells.Lane 4: protein extract of wild type P0-GFP-V5 transfected cells.Lane 5: moelcular weight standard.