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Table 1 Summary of Galectin-1 Mutations

From: Partial identification by site-directed mutagenesis of a cell growth inhibitory site on the human galectin-1 molecule

Mutation Yield (mg/l culture) Haemagglutination. Endpoint (μg/ml) Growth Inhibition I50 (μg/ml)
None 8.0 1 5
R49G* 0.1 1 nd
N47D* 0.1 100 nd
A28R* 0.3 - >200
D103A 9.2 1 20
P79R 0.6 500 4
C131S 0.5 - 13
K29M 8.0 1 -
K29T 8.2 1 -
D27N 0.8 1 170
M1R 10.4 1 -
M1R (thrombin) n/a 1 24
  1. Yields and Biological Activities of Recombinant Galectin-1 Derivatives For each mutation in galectin-1, the yield of protein per litre of bacterial culture, the endpoint of the haemagglutination titration (in μg/ml) and the concentration giving 50% inhibition of fibroblast growth (I50, in μg/ml) are listed. The three entries marked * are for low-yield mutations, for which full analysis was not feasible. The final row contains data for the M1R mutant following thrombin cleavage and repurification.