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Table 1 Summary of Galectin-1 Mutations

From: Partial identification by site-directed mutagenesis of a cell growth inhibitory site on the human galectin-1 molecule

Mutation

Yield (mg/l culture)

Haemagglutination. Endpoint (μg/ml)

Growth Inhibition I50 (μg/ml)

None

8.0

1

5

R49G*

0.1

1

nd

N47D*

0.1

100

nd

A28R*

0.3

-

>200

D103A

9.2

1

20

P79R

0.6

500

4

C131S

0.5

-

13

K29M

8.0

1

-

K29T

8.2

1

-

D27N

0.8

1

170

M1R

10.4

1

-

M1R (thrombin)

n/a

1

24

  1. Yields and Biological Activities of Recombinant Galectin-1 Derivatives For each mutation in galectin-1, the yield of protein per litre of bacterial culture, the endpoint of the haemagglutination titration (in μg/ml) and the concentration giving 50% inhibition of fibroblast growth (I50, in μg/ml) are listed. The three entries marked * are for low-yield mutations, for which full analysis was not feasible. The final row contains data for the M1R mutant following thrombin cleavage and repurification.
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BMC Molecular and Cell Biology

ISSN: 2661-8850

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